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Enhanced Neuroimaging Core


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Thanks to a major new grant, we are pleased to announce a major expansion of the program. Furthermore, as of November 2012 the Enhanced Neuroimaging Core will be open to investigators from beyond the Harvard community. Please refer to our revised “Access” guidelines below for further details.




The Enhanced Neuroimaging Core provides access to advanced confocal, multi-photon and wide field microscopy services. Comprehensive training in data acquisition and image analysis are offered as customized services to new and established users throughout the NeuroDiscovery community. Please contact This e-mail address is being protected from spam bots, you need JavaScript enabled to view it , the optical imaging manager, or This e-mail address is being protected from spam bots, you need JavaScript enabled to view it , the optical imaging specialist to discuss your imaging needs.

Program Resources

Instrumentation

A Leica SP8 X inverted confocal microscope, a Zeiss LSM 510 META upright confocal microscopes and a Yokogawa Spinning Disk microscope are currently available within the facility.

The LSM 510 microscope has three visible wavelength lasers and a META spectral emission detector.  It has a Coherent Chameleon™ Multi-photon Excitation (MPE) laser tunable from 720nm – 930nm with 4 NLOs (External detectors). The Chameleon™ provides widely tunable femtosecond pulses with more than 1W of average power.

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Olympus/Andor/Yokogawa Spinning Disk Microscope
Leica SP8 X Confocal Microscope

Peripheral Equipment
• VWR tissue culture incubator (37°C w/ 5% CO2) for short-term storage.

Workstations

• Dell Optiplex 980 2.93 GHz processor (4 cores, w/ HT), 16 GB RAM and 512 MB ATI Radeon HD 4550
• Dell Precision 380 3.73 GHz processor (2 cores, w/ HT), 3.5 GB RAM, and 512 MB Nvidia QuadroFX 4500
• Dell Precision 380 3.00 GHz processor (2 cores, w/o HT), 3.5 GB RAM, and 512 MB Nvidia QuadroFX 4400

Image Analysis

Imaris Bitplane - Easily work in 3D! Imaris loads, processes, visualizes, and measures a range of multi-dimensional images.  Results are displayed with advanced movie generation and export functions. The facility owns a license to Imaris Suite 5.5, FilamentTracer 5.5, Imaris XT and AutoAligner 2.0.

Metamorph Imaging System - MetaMorph's extensive analytical functions can measure areas and distances, count cells, graph intensity levels over time, deconvolve images, or color combine multi-fluorescence images.

Metafluar Offline- Software is designed for dual-wavelength intracellular ion measurements.

SVI Huygens Professional - Restore images and reduce out of focus blur by removing the PSF effect. 

Zeiss AIM - Create overlays, projections, and export images.

Viewing Images

LSM Image Browser - a free Windows program for viewing, manipulating and exporting Zeiss LSM Files. 

FocalPoint Image Viewer - a thumbnail viewer for Windows, Mac, and Linux that generates thumbnails from .lsm files.

ImageJ - a free NIH program for Macs, PCs and Linux. (ImageJ) (MBF_ImageJ)

NOTE: We are actively investigating a number of new instruments. Investigators are encouraged to make recommendations regarding specific equipment that may be useful to the research community. Please contact the This e-mail address is being protected from spam bots, you need JavaScript enabled to view it with questions or suggestions.

Access

Although the facility is primarily for the Harvard and Harvard-affiliated community, starting November 2012 we will also accept users from other Massachusetts universities and research institutions.

When demand outstrips supply, we will prioritize access based on a combination of the relevance of the projects to the neurobiological, neurological and neuropsychiatric mission of the Center, and the source of the funds supporting the core.

In practice this is likely to mean that whereas the bulk of our users will continue to be from the wider Harvard-affiliate community, there will also be considerable access for non-Harvard, Massachusetts investigators.

Please contact This e-mail address is being protected from spam bots, you need JavaScript enabled to view it , the optical imaging manager, or This e-mail address is being protected from spam bots, you need JavaScript enabled to view it , the optical imaging technician to discuss your imaging needs.

If you need training or access to instruments, please complete our online user form. After submitting the User Form you will be contacted to schedule a training session. All users must complete training to gain access to the facility.

The facility is staffed Monday-Friday, 8:00 am-5:00 pm. Experienced users are provided access outside of these regular hours. Only those users with sufficient training on the microscopes can gain access to the facility during off-peak hours.