ATRC Library
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Laser Capture Microscopy publications:
- Decreased levels of BDNF protin in Alzheimer temporal cortex are independent of BDNF polymorphisms.
- Transcriptional dysregulation in striatal projection - and interneurons in a mouse model of Huntington's disease: neuronal selectivity and potential neuroprotective role of HAP1
- Proteomic analysis of neurofibrillary tangles in Alzheimer disease identifies GAPDH as a detergent-insoluble paired helical filament tau binding protein
- Improved Detection of EGFP in Transgenic Mice
- Affymetrix 2000 - BioTechniques LCM and array hybridization
- Alevizos 2001 - LCM & Expression Profiling
- Ball 2002 - Isolation of brain vasculature by LCM
- Baugh 2001 - Quantitative Analysis of mRNA Amplification by In Vitro Transcription
- Bi 2002 - Identification of Nitric Oxide Synthase Neurons for Laser Capture Microdissection and mRNA Quantification
- Bonaventure 2002 - LCM and expression profiling in mammalian brain
- Dolter 2002 - Small sample total RNA purification
- Elkahloun 2002 - In Situ Gene Expression Analysis of Cancer Using Laser Capture Microdissection, Microarrays and Real Time Quantitative PCR
- Fink 2000 - Immunostaining for Cell Picking and Real-Time mRNA Quantitation
- Fink 2002 - cDNA Array Hybridization after Laser Assisted Microdissection from Nonneoplastic Tissue
- Gillespie 2002 - Non-formalin tissue fixation for molecular profiling
- Lehmann 2003 Formalin fixed RNA and DNA extraction
- Luzzi 2003 - Accurate and Reproducible Gene Expression Profiles from Laser Capture Microdissection, Transcript Amplification, and High Density Oligonucleotide Microarray Analysis
- Vincent 2002 - Analysis of Neuronal Gene Expression with Laster Caputure Microdissection
- Xu 2002 - Direct Analysis of Laser Capture Microdissected Cells by MALDI Mass Spectrometry
- Springer 2003 - Single cell splice variant detection via LCM of neurons
- Laser microdissection of immunolabeled astrocytic gene expression
- Preparation of human heart for laser microdissection and proteomics
Laser Capture Microscopy protocols:
- Using DSP, a reversible cross-linker, to fix tissue sections for immunostaining, microdissection and expression profiling
- Rapid Brain Vasculature Staining for LCM
- Paradise Reagent Kit Protocol
- Retinal Prep for LCM
- aRNA Amplification
- Immunhistochemical staining protocol
- Lipofuscin Autoflourescence Reduction
- Sudan Black for LCM
- Proteinase K Digestion
- Hematoxylin and Eosin Staining
- Laser Capture Microdissection of Living in vitro Cells
Real Time PCR protocols:
- ICycler IQ User Manual
- Relative Expression Calculations
- Relative Expression Calculations Using Pfaffl Method
- Relative Expression Calculations
- Relative Gene ExpressionUsing DeltaDelta Ct Method
- SYBR Green Reaction Mix Calculations
Real Time PCR publications:
- A new mathematical model for relative quanitfication in real-time RT-PCR
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-ΔΔCT method
- Housekeeping Genes as internal standards- use and limits
- Housekeeping Genes
- Human immunoglobulin variable region gene analysis by single cell RT-PCR
- Presence of Dendritic Cells, MCP-1, and Activated Microglia/Macrophages in Amyotrophic Lateral Sclerosis Spinal Cord Tissue
- Real-Time Good Review Articles
- Real-Time PCR Analysis of DNA and RNA Extracted from Formalin-Fixed and Paraffin-Embedded Biopsies
- Relative Expression Calculations
Agilent Bioanalysis publications:
- PICO RNA analysis from LCM
- Character of RNA quality using the Agilent 2100 Bioanalyzer
- High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip® kits
Agilent Bioanalysis protocols:
- Reagent Kit Guide DNA 500 and 1000 Assay
- Reagent Kit Guide Protein 200 Plus Assay
- Reagent Kit Guide RNA 6000 Nano Assay
- Reagent Kit Guide RNA 6000 Pico Assay